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Jingdong book

高效液相色谱在中药研究中的应用

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高效液相色谱在中药研究中的应用

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Content Description

《高效液相色谱在中药研究中的应用》系统地介绍了高效液相色谱法(HPLC)和高效液相色谱仪的组成,重点介绍了高效液相色谱在中药热点研究领域中的应用,即在中药有效成分的含量测定、中药指纹图谱和中药代谢动力学研究中的应用。全书共七章,主要内容包括高效液相色谱仪、检测器、样品预处理、HPLC在中药有效成分含量测定中的应用、HPLC在中药指纹图谱研究中的应用、HPLC在中药药代动力学研究中的应用等。
《高效液相色谱在中药研究中的应用》可供从事高效液相色谱分析工作和中药研究的技术人员学习参考.也可作为高等院校相关专业师生的参考书。
Catalogue

1 绪论
1.1 高效液相色谱法的特点
1.1.1 高效液相色谱法发展简史
1.1.2 与经典液相色谱法比较
1.1.3 与气相色谱法比较
1.1.4 高效液相色谱法的应用范围
1.1.5 高效液相色谱法的优点
1.2 常用术语
1.3 高效液相色谱法分离原理与分类
1.3.1 液-固吸附色谱法
1.3.2 液-液分配色谱法
1.3.3 离子色谱法
1.3.4 凝胶色谱法
1.3.5 亲和色谱法
1.3.6 超临界色谱法
参考文献
2 高效液相色谱仪
2.1 输液系统
2.1.1 储液瓶
2.1.2 高压输液泵
2.1.3 梯度洗脱装置
2.1.4 输液系统的辅助设备
2.2 进样系统
2.2.1 隔膜注射进样装置
2.2.2 六通进样阀
2.2.3 自动进样器
2.3 分离系统
2.3.1 色谱柱的结构
2.3.2 色谱柱的填料与填充技术
2.3.3 色谱柱的保护
2.3.4 色谱柱的评价
2.3.5 色谱柱的保存
2.3.6 色谱柱的再生
参考文献
3 检测器
3.1 检测器的分类
3.2 检测器的性能指标
3.2.1 噪声
3.2.2 漂移
3.2.3 灵敏度
3.2.4 敏感度
3.2.5 线性范围
3.3 常用检测器
3.3.1 紫外.可见检测器
3.3.2 示差折光检测器
3.3.3 电化学检测器
3.3.4 荧光检测器
3.3.5 蒸发光散射检测器
3.3.6 化学发光检测器
3.3.7 联用仪器
参考文献
4 样品预处理
4.1 生物样品采集、制备与贮存
4.1.1 血液的采集、制备与贮存
4.1.2 尿液的采集与贮存
4.2 中药样品的提取和分离
4.2.1 溶剂提取法
4.2.2 膜分离法
4.3 固相萃取
4.3.1 固相萃取的原理
4.3.2 固相萃取的装置
4.3.3 固相萃取的一般步骤
4.3.4 固相萃取特点
4.3.5 固相萃取应用
4.4 超临界流体萃取
4.4.1 超临界流体萃取的原理
4.4.2 超临界流体萃取的特点
4.4.3 超临界流体萃取技术的应用
参考文献:
5 PLC在中药有效成分含量测定中的应用
5.1 生物碱类成分的含量测定
5.1.1 吴茱萸中吴茱萸碱和吴茱萸次碱的含量测定
5.1.2 三颗针中四种生物碱的含量测定
5.1.3 板蓝根中两种生物碱的含量测定
5.1.4 紫金龙中四种异喹啉类生物碱的含量测定
5.2 皂苷类成分的含量测定
5.2.1 甘草中甘草酸的含量测定
5.2.2 白英中薯蓣皂苷元的含量测定
5.2.3 黄芪桂枝五物汤中的黄芪甲苷的含量测定
5.2.4 益心复脉颗粒中人参皂苷Rgl的含量测定
5.3 蒽醌类成分的含量测定
5.3.1 丹参中丹参酮ⅡA的含量测定
5.3.2 大黄中五种蒽醌类衍生物的含量测定
5.3.3 消炎止血颗粒中大黄素和大黄酚的含量测定
……
6 HPLC在中药指纹图谱研究中的应用
7 HPLC在中药药代动力学研究中的应用
参考文献
Book Abstract

1.1.5高效液相色谱法的优点
通过高效液相色谱法与经典液相色谱法、气相色谱法的比较,可以发现高效液相色谱法是汲取了经典液相色谱法和气相色谱法的优点,综合起来高效液相色谱法具有以下优点:
(1)高效。高效液相色谱法分离效率极高,柱效一般可达每米10。理论塔板数。使用微型填充柱和毛细管液相色谱柱时,分析样品柱效可达到每米十几万理论塔板数。
(2)高速。由于使用了高压输液泵、梯度洗脱和柱后直接检测等手段,使其流速可达到0.1~10.0m/min。通常分析一个样品在15~30min,有些样品甚至在5min内即可完成。
(3)高分辨率。可选择固定相和流动相以达到最佳分离效果。一根长10cm的柱子可以分离十几种以上的物质。
(4)高灵敏度。紫外检测器灵敏度可达0.01ng,荧光和电化学检测器可达0.1Pg,,
(5)适用范围广,灵活性强。几乎所有的生物大分子根据它们的性质差别(等电点、疏水性、相对分子质量、电荷分布等)都可以使用不同的HPLC方法进行分离提纯,在一定的条件下,还可以保持其高的生物学活性,且极易回收。
另外,高效液相色谱柱可反复使用,用一根色谱柱可分离不同的化合物。而且样品量少,容易回收,样品经过色谱柱后不被破坏,可以收集单一组分或做制备,重复性好。
Introduction

中药是中华民族文化宝库中的瑰宝,是我国人民数千年来实践经验的总结。中药产业是我国传统产业,也是颇具优势和特色的产业。目前,世界中药市场总价值已超过600亿美元,且每年还在以10%的速度增长。作为传统中药大国,要使我国中药与国际水平接轨,被世界所认同和接受,提高我国中药产业在世界市场的份额,就必须实现中药现代化。中药现代化的主要研究内容是采用现代先进科学技术,明确中药的有效成分,阐明作用机理,使中药的质量控制、剂型和生产工艺等均符合国际标准。中药成分分析、中药指纹图谱和中药代动力学研究是中药现代化的基础和重要的组成部分。
由于高效液相色谱法具有灵敏度高、选择性好、分离性能好、分析速度快、适用范围广、样品用量少、安全、可根据被检样品的理化特性搭配适当的检测器等优点,已成为中药成分分析、中药指纹图谱和中药代动力学研究的重要手段。
近年来,高效液相色谱在中药研究中的应用得到了迅速发展,因此,本书在内容安排上,除重点介绍高效液相色谱法和高效液相色谱仪外,还着重介绍了高效液相色谱在中药热点研究领域中的应用,即在成分分析、指纹图谱、药代动力学研究中的应用。

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