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Jingdong book

免疫学实验技术

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Books Medical Science Basic Medicine

Jingdong book

免疫学实验技术

Name: 免疫学实验技术
Brand: Jingdong book
SKU: 3117086191
Price: 22.43 USD
Availability: OutOfStock

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Content Description

《免疫学实验技术》系统地介绍了免疫学实验技术的基础知识、基本理论和基本技能。全书共分16个实验，内容包括凝集反应、沉淀反应、补体参与的免疫反应、免疫标记技术、免疫印迹、免疫细胞的分离与纯化、细胞免疫功能测定、流式细胞测定技术、细胞分子及其受体的检测、化学发光免疫分析、HLA分型技术、超敏反应、多克隆抗体的制备、单克隆抗体的制备、抗体的纯化和非特异性免疫实验等。书中详细介绍了上述实验的原理、材料、方法及实验注意事项，内容丰富、知识性强。《免疫学实验技术》可作为基础医学、临床医学、口腔医学、预防医学、护理学、药学及生物学等专业的免疫学实验教材，同时也可作为从事免疫学及相关研究人员的参考用书。

Catalogue

实验室守则
实验一凝集反应
一、直接凝集反应
（一）玻片凝集试验——ABO血型鉴定
（二）试管凝集试验
二、间接凝集试验
（一）间接血球凝集试验——血清类风湿因子测定
（二）间接凝集抑制试验——妊娠试验
（三）协同凝集试验

实验二沉淀反应
一、琼脂扩散试验
（一）单向琼脂扩散试验
（二）双向琼脂扩散试验
二、免疫电泳试验
（一）对流免疫电泳试验
（二）免疫电泳试验
（三）火箭免疫电泳试验
（四）交叉免疫电泳试验
三、环状沉淀试验

实验三补体参与的免疫反应
一、溶血反应
二、补体结合试验
（一）溶血素单位滴定
（二）补体单位滴定
（三）正式试验
三、血清总补体含量的测定（CH50测定）
四、溶血空斑试验
五、补体介导的细胞毒试验

实验四免疫标记技术
一、酶免疫技术
（一）酶联免疫吸附试验
（二）酶免疫组织化学技术-PAP法（可溶性酶抗酶法）
二、荧光免疫技术
（一）直接（或间接）荧光免疫法检测标本中的抗原
（二）间接荧光免疫法检测人外周血中T淋巴细胞亚群
三、金免疫技术
（一）胶体金的制备
（二）免疫金的制备
（三）斑点免疫渗滤试验
（四）斑点免疫层析试验
四、放射免疫技术

实验五免疫印迹
一、蛋白质的电泳分离
（一）样本的制备
（二）蛋白质凝胶电泳
二、将蛋白质从凝胶中转印至膜上
（一）以PVDF膜为固相载体的半干转印法
（二）转印后切取印迹膜
（三）印迹膜蛋白染色
三、免疫检测
（一）印迹膜上非特异性蛋白质结合位点的封闭
（二）直接与间接检测方法

实验六免疫细胞的分离与纯化
一、外周血液中白细胞的分离
（一）自然沉降法
（二）高分子聚合物沉降法
二、外周血液中单个核细胞的分离——密度梯度离心法
三、淋巴组织中淋巴细胞的分离
（一）脾细胞悬液的制备
（二）淋巴结细胞悬液的制备
（三）胸腺细胞悬液的制备
四、淋巴细胞的分离纯化
（一）分离PMNC中的淋巴细胞和巨噬细胞
（二）T细胞、B细胞及T细胞亚群的分离纯化
……
实验七细胞免疫功能测定
实验八流式细胞测定技术
实验九细胞因子及其受体的检测
实验十化学发光免疫分析
实验十一 HLA分型技术
实验十二超敏反应
实验十三多克隆抗体的制备及纯化
实验十四单克隆抗体的制备
实验十五抗体的纯化
实验十六非特异性免疫实验
附录

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Jingdong book

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