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Jingdong book

流行病学实习指导(第3版)

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流行病学实习指导(第3版)

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Content Description

《流行病学实习指导(第3版)》编写以突出“三基”为主,注重培养学生独立思考能力、知识创新能力和实际工作能力,书中除编排少量必须掌握的基础指标外,大多数以典型的流行病学研究实例为课题进行讨论分析,让学生掌握流行病学的思维方式。此外,还增加了实验内容,培养学生实验操作能力。《流行病学实习指导(第3版)》共设19个实习内容,可供预防、临床、口腔医学等专业使用,另外,还可以作为非预防专业研究生的流行病学课程实习用书。
Catalogue

第3版前言
第1版前言
实习1 疾病频率的测量
实习2 疾病的分布
实习3 现况调查
实习4 筛查及诊断试验的评价
实习5 病例对照研究
实习6 队列研究
实习7 偏倚及其控制
实习8 病因未明疾病的调查
实习9 预防接种效果评价
实习10 爆发调查
实习11 遗传流行病学的应用
实习12 血清流行病学
实习13 分子流行病学
实习14 消毒及效果评价
实习15 疾病监测
实习16 临床试验设计
实习17 医学文献的评阅与写作
实习18 Epidata 3.1软件在流行病学中的应用
实习19 Review Manager 5.0软件在Meta分析中的应用
附录
复习思考题
复习思考题参考答案
参考文献
Book Abstract

问题2.1 本次实验分组的目的是什么?
问题2.2 阳性和阴性对照为什么用中和产物溶液,而不以PBS或生理盐水代替消毒液?
问题2.3 请对本次试验结果进行解释?
问题2.4 请分析影响试验结果的因素有哪些?你是如何进行质量控制的?
2.细菌定量杀灭试验
目的在实验室内测定消毒剂杀灭载体上细菌繁殖体所需剂量,以作为制定实用消毒剂量的依据。
主要试剂和器材①制备大肠杆菌菌片;②消毒剂溶液除有特殊规定者外,应使用蒸馏水配制。消毒剂溶液浓度应以所含有效成分为准。例如,含氯消毒剂以所含有效氯浓度为准,碘伏以所含有效碘为准,过氧乙酸以所含过氧乙酸量为准,复方消毒剂浓度以主要杀菌有效成分的量为准。各组消毒剂溶液有效成分浓度的计算,应以菌药混合液中有效成分的最终浓度为准;③去除残留消毒剂的中和剂或设备;④磷酸盐缓冲液(PBS,0.03mol/L,pH7.2 );⑤营养琼脂培养基;⑥刻度吸管(O.1 ml、l.0ml、5.0m1);⑦恒温水浴箱;⑧喷雾器。
试验分组①试验组中选定3个消毒剂浓度和4个作用时间进行试验(共12个剂量)。从所定12个剂量的结果,应测出杀灭99.9 0%和100.00%所需的下限剂量(即最低有效剂量,包括浓度和作用时间)。必要时可先进行预试验,选出适宜的测试浓度和作用时间(作用时间应不少于30s)。初选时组距可大些,然后再以有效组为基础缩小上下组距做进一步试验,以测出更为准确的最低有效剂量;②阳性对照组,根据各种试验的规定,用PBS代替消毒剂溶液,按载体喷雾定量杀灭试验的操作步骤进行试验,所得结果代表菌液原有浓度,以其作为基数进行杀灭率计算;③阴性对照组,以本次用后剩余的中和剂、:PBS培养基作为阴性对照组。将上述中和剂、PBS接种营养琼脂平板进行培养,将未种菌的同批次培养基直接放入温箱中培养,以确定所用上述试液和培养基有无污染。
……
Introduction

《流行病学实习指导》出版迄今已快十年了,印数不断增加,深受使用单位和各校师生的喜爱。与此同时,读者和相关专家、授课教师也对本书提出了许多宝贵意见和建议,作为主编甚为欣慰和高兴!
该书出版的十年来,我们遇到了过去50年来从未遇到过的众多重大公共卫生问题,例如SARS的突然出现,使流行病学得到了空前的高度重视,我国的预防医学尤其是流行病学取得了长足的进步和发展。同时,我们安徽医科大学流行病学学科也取得了一定的成绩。2003年,我们取得了流行病与卫生统计学博士学位授权点;2005年,《深化教学改革,提高医学生预防控制疾病能力的研究》获得国家级教学成果二等奖;2007年,“流行病学”成为安徽医科大学第一个国家级精品课程,《流行病学实习指导》是其中一个重要的组成部分。回想十年前,我们几所学校中青年老师聚在一起,商讨编写一本既适合本校特点,又能贴近实际预防工作的实习指导,从而能培养出新时期的预防医学工作所需的高级人才。所有这些,充分体现《流行病学实习指导》教材是一本融实用性、操作性和可读性于一体的精品教材。
针对使用单位和学者所提的问题和建议,结合流行病学近年来的进展和教学中存在的问题,我们决定对该书部分内容进行修改和补充。第3版仍基本保持第1版和第2版的体系、结构。全书共设19个实习内容,前17章仍保留第1版和第2版的实习章节,但已对大部分的内容进行了修改和完善。为适应新时期流行病学现场工作的需求,将原第18章的“EpiInfo软件在流行病学中的应用”改为“Epidata 3.1软件在流行病学中的应用”,并增加了第19章“Review Manager 5.O软件在Meta分析中的应用”。

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